Format Conversion

Convert between formats such as. fasta. gff, Genbank, fna,. faa, etc.

convert
Average Nucleotide Identity (ANI)
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Drop files here or click to upload

Please note that uploaded files must inFASTA format, and file suffixes only accept.fna, .fa, .fasta.
Upload two or more DNA sequence file(s) in FASTA format to start the analysis
Tips: ANIm relies on whole-genome alignments, which may fail when comparing distantly related organisms (e.g., inter-genera comparisons). ANIb are preferred in such cases.
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Application Information
More Info

Using pyani to compute the genomic-level similarity of genomes

Reference

如果您在研究中使用了本工具，请在文章中 引用下列文章:

TracePatho. 2025. TracePatho: All-in-one microbial analysis platform. Accessed July 9, 2025. https://tracepatho.com/.

Pritchard et al. Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens. Anal Methods, 2016,8:12-24. doi:10.1039/C5AY02550H

Goris, Johan, et al. DNA-DNA Hybridization Values and Their Relationship to Whole-Genome Sequence Similarities. International Journal of Systematic and Evolutionary Microbiology, vol. 57, no. 1, 2007, pp. 81-91. Microbiology Society, doi:10.1099/ijs.0.64483-0.