Basic Info

Analyze the mutation status of common loci A2063G, A2064G, C2617G, and A2067G within the drug-resistance genes of Mycoplasma pneumoniae

More Details

Using Snippy v4.6.0 software, we aligned the reference genome of Mycoplasma pneumoniae M129-B7 (CP003913.2) with the uploaded sequences. Subsequently, converted alignment results to VCF Format using the faToVcf tool. By utilizing the vcftools v0.1.16 software, we screened the VCF file for variation information related to the four known drug-resistance-associated loci: A2063G, A2064G, C2617G, and A2067G. Then analyzed and annotated the variation types of the uploaded sequences, obtaining the variation results for the drug-resistance-associated loci.

What's New

NA

Additional Information

Related links:

https://vcftools.github.io/

http://hgdownload.soe.ucsc.edu/admin/exe/

https://github.com/tseemann/snippy

Literature

Sun Y, Li P, Jin R, Liang Y, Yuan J, Lu Z, et al. Characterizing the epidemiology of Mycoplasma pneumoniae infections in China in 2022–2024: a nationwide cross-sectional study of over 1.6 million cases. J Abbrev. 2025;14(1).doi: 10.1080/22221751.2025.2482703.

Wang YS, Zhou YL, Bai GN, et al. Expert consensus on the diagnosis and treatment of macrolide-resistant Mycoplasma pneumoniae pneumonia in children. World J Pediatr. 2024;20(9):901-914. doi:10.1007/s12519-024-00831-0