Basic Info

Serotype prediction based on their surface antigens, such as O, H, and K antigens.

More Details

The serotype represents the genetic markers displayed by proteins in blood plasma. Due to the genetic polymorphism of some serum proteins, phenotypic differences arise among individuals. Therefore, serotyping has become an important classification method, especially for many foodborne microorganisms such as Salmonella and Listeria monocytogenes. This method is also widely used in epidemiological investigations. By determining the serotype of bacteria, we can provide a solid basis for antimicrobial therapy. Specifically, through in-depth analysis of the characteristics of bacterial somatic cells, such as the O antigen, cell surface proteins, H antigen, and K antigen, we can accurately classify bacteria based on their serotypes.

What's New

The Providencia genus was originally classified into 63 O serotypes by specific agglutination reactions with adsorbed antisera, with O58 and O59 later re-classified to Morganella morganii, thus leaving 61 O serotypes remaining. To date, the 23 serotypes included O3, O6, O8, O9, O12, O19, O20, O22, O28-O31, O33, O34, O36, O38, O40, O44-O48, and O52 were identified, and each O-antigen gene cluster region was analyzed. A PCR-based suspension array was developed to distinguish these 23 serotypes based on sero-specific gene diversity.

Additional Information

NA

Literature

Du Y, Li H, Yin Z, et al. Development of a molecular serotyping scheme and a multiplexed luminex-based array for Providencia. J Microbiol Methods. 2018;153:14-23. doi:10.1016/j.mimet.2018.08.009

Ovchinnikova OG, Rozalski A, Liu B, Knirel YA. O-antigens of bacteria of the genus providencia: structure, serology, genetics, and biosynthesis. Biochemistry (Mosc). 2013;78(7):798-817. doi:10.1134/S0006297913070110

Liu B, Chen M, Perepelov AV, et al. Genetic analysis of the O-antigen of Providencia alcalifaciens O30 and biochemical characterization of a formyltransferase involved in the synthesis of a Qui4N derivative. Glycobiology. 2012;22(9):1236-1244. doi:10.1093/glycob/cws089

Ovchinnikova OG, Liu B, Guo D, et al. Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. FEMS Immunol Med Microbiol. 2012;66(3):382-392. doi:10.1111/1574-695X.12002