Basic Info

Serotype prediction based on their surface antigens, such as O, H, and K antigens.

More Details

The serotype represents the genetic markers displayed by proteins in blood plasma. Due to the genetic polymorphism of some serum proteins, phenotypic differences arise among individuals. Therefore, serotyping has become an important classification method, especially for many foodborne microorganisms such as Salmonella and Listeria monocytogenes. This method is also widely used in epidemiological investigations. By determining the serotype of bacteria, we can provide a solid basis for antimicrobial therapy. Specifically, through in-depth analysis of the characteristics of bacterial somatic cells, such as the O antigen, cell surface proteins, H antigen, and K antigen, we can accurately classify bacteria based on their serotypes.

What's New

The O-antigen constitutes approximately 15% of the lipopolysaccharide (LPS) molecule in Pseudomonas aeruginosa. Based on variations in the O-antigen, the International Antigenic Typing System (IATS) classifies 20 serotypes (O1-O20) in this pathogen. Within each serotype, subtle structural variations of the O-antigen among substrains cumulatively define >30 subtypes. A single P. aeruginosa strain may co-express two different structural and serotype variations of the O-antigen. Notably, not all strains of Pseudomonas aeruginosa express the O-antigen; many strains that cause chronic Pseudomonas aeruginosa infections do not express the O-antigen.

Additional Information

NA

Literature

Magalhães B, Valot B, Abdelbary MMH, et al. Combining Standard Molecular Typing and Whole Genome Sequencing to Investigate Pseudomonas aeruginosa Epidemiology in Intensive Care Units. Front Public Health. 2020;8:3. Published 2020 Jan 28. doi:10.3389/fpubh.2020.00003

Shoumali L, Masoud H, Khlaif H, Migdadi H, Masoud S. Serologic and molecular characterization of Pseudomonas aeruginosa Jordanian clinical isolates compared with the strains of International Antigenic Typing Scheme. Diagn Microbiol Infect Dis. 2007;58(4):393-398. doi:10.1016/j.diagmicrobio.2007.03.005

Li H, Du Y, Qian C, et al. Establishment of a suspension array for Pseudomonas aeruginosa O-antigen serotyping. J Microbiol Methods. 2018;155:59-64. doi:10.1016/j.mimet.2018.11.006