Basic Info

Serotype prediction based on their surface antigens, such as O, H, and K antigens.

More Details

The serotype represents the genetic markers displayed by proteins in blood plasma. Due to the genetic polymorphism of some serum proteins, phenotypic differences arise among individuals. Therefore, serotyping has become an important classification method, especially for many foodborne microorganisms such as Salmonella and Listeria monocytogenes. This method is also widely used in epidemiological investigations. By determining the serotype of bacteria, we can provide a solid basis for antimicrobial therapy. Specifically, through in-depth analysis of the characteristics of bacterial somatic cells, such as the O antigen, cell surface proteins, H antigen, and K antigen, we can accurately classify bacteria based on their serotypes.

What's New

Aldová et al. recognized 102 O-antigens and 51 H-antigens of Plesiomonas Shigelloides (P. shigelloides). The P. shigelloides strain 302-73, originally isolated in Japan, was determined and named as the ‘O1 representative strain.’ In 2010, the complete chemical structures of its O-antigen and lipopolysaccharide core were characterized. Based on the analysis of 21 O-antigen clusters of P. shigelloides (serogroups O2, O8, O10, O12, O17, O18, O23, O25, O26, O32, O33, O34, O37, O38, O39, O44, O45, O61, O66, O75, and O76), it became clear that the O-antigen gene clusters group together and are located on the chromosome between the rep and aqpZ genes. Among these 21 serogroups, 17 use the Wzx/Wzy pathway to synthesize and translocate O chains, and the other four use the wzm/wzt-encoded ATP-binding cassette (ABC) transporter pathway.

Additional Information

NA

Literature

Wang X, Xi D, Li Y, et al. Genetic Analysis and Serological Detection of Novel O-Antigen Gene Clusters of Plesiomonas shigelloides. J Microbiol Biotechnol. 2021;31(4):520-528. doi:10.4014/jmb.2010.10008

Xi D, Wang X, Ning K, et al. O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme. Front Microbiol. 2019;10:741. Published 2019 Apr 10. doi:10.3389/fmicb.2019.00741

Aldová E, Shimada T. New O and H antigens of the international antigenic scheme for Plesiomonas shigelloides. Folia Microbiol (Praha). 2000;45(4):301-304. doi:10.1007/BF02817550

Pieretti G, Carillo S, Lindner B, et al. The complete structure of the core of the LPS from Plesiomonas shigelloides 302-73 and the identification of its O-antigen biological repeating unit. Carbohydr Res. 2010;345(17):2523-2528. doi:10.1016/j.carres.2010.09.007