The serotype represents the genetic markers displayed by proteins in blood plasma. Due to the genetic polymorphism of some serum proteins, phenotypic differences arise among individuals. Therefore, serotyping has become an important classification method, especially for many foodborne microorganisms such as Salmonella and Listeria monocytogenes. This method is also widely used in epidemiological investigations. By determining the serotype of bacteria, we can provide a solid basis for antimicrobial therapy. Specifically, through in-depth analysis of the characteristics of bacterial somatic cells, such as the O antigen, cell surface proteins, H antigen, and K antigen, we can accurately classify bacteria based on their serotypes.
Listeria monocytogenes (LM) is an opportunistic foodborne pathogen responsible for listeriosis in humans. It is capable of surviving in a wide range of natural environments due to the ability to tolerate low temperatures, low pH values, and salt concentrations. LM can be divided into 14 serotypes. The predominant serotypes responsible for over 90 % of human listeriosis cases are 1/2a, 1/2b, and 4b, which have consistently been identified in numerous fresh produce-associated listeriosis outbreaks.
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