Basic Info

Serotype prediction based on their surface antigens, such as O, H, and K antigens.

More Details

The serotype represents the genetic markers displayed by proteins in blood plasma. Due to the genetic polymorphism of some serum proteins, phenotypic differences arise among individuals. Therefore, serotyping has become an important classification method, especially for many foodborne microorganisms such as Salmonella and Listeria monocytogenes. This method is also widely used in epidemiological investigations. By determining the serotype of bacteria, we can provide a solid basis for antimicrobial therapy. Specifically, through in-depth analysis of the characteristics of bacterial somatic cells, such as the O antigen, cell surface proteins, H antigen, and K antigen, we can accurately classify bacteria based on their serotypes.

What's New

An O serotyping scheme has been established for Vibrio Fluvialis (V. fluvialis), which has a total of 51 O-serogroups. Among them, O1–O18 are prevalent serogroups which are frequently isolated from clinical and environmental isolates. Currently, no commercial antisera for V. fluvialis are available, and thus the majority of laboratories are unable to perform conventional antigenic typing analysis for V. fluvialis. In 2022, Wang M, et al. conducted whole-genome sequencing analysis of 18 V. fluvialis serogroups (O1–O18) , revealing highly diverse O-antigen gene clusters. They developed a microsphere-based suspension array targeting O-serogroup-specific genes, which demonstrated high specificity and sensitivity in double-blind validation for strain identification. Furthermore, genomic analysis of 62 publicly available V. fluvialis strains identified 13 novel O-antigen gene cluster types (new1-new13).

Additional Information

NA

Literature

Wang M, Liu B, Xu Y, et al. Characterization of the O-antigen gene clusters and development of a molecular serotyping method for Vibrio fluvialis. Int J Food Microbiol. 2022;370:109665. doi:10.1016/j.ijfoodmicro.2022.109665

Shimada T, Sakazaki R. Serological studies on Vibrio fluvialis. Jpn J Med Sci Biol. 1983;36(6):315-323. doi:10.7883/yoken1952.36.315